Sutezolid Synthesis Essay

INTRODUCTION TO SYNTHESES

(mostly from Cassie Carter - with her kind permission)


CONTENTS
What is a synthesis?
Two types of syntheses
Standards for synthesis essays
How to write synthesis essays
Techniques for developing synthesis essays
Thesis statements, introductions, conclusions, and quotations

WHAT IS A SYNTHESIS?
A synthesis is a written discussion that draws on one or more sources. It follows that your ability to write syntheses depends on your ability to infer relationships among sources - essays, articles, fiction, and also nonwritten sources, such as lectures, interviews, observations. This process is nothing new for you, since you infer relationships all the time - say, between something you've read in the newspaper and something you've seen for yourself, or between the teaching styles of your favorite and least favorite instructors. In fact, if you've written research papers, you've already written syntheses. In an academic synthesis, you make explicit the relationships that you have inferred among separate sources.

The skills you've already been practicing in this course will be vital in writing syntheses. Clearly, before you're in a position to draw relationships between two or more sources, you must understand what those sources say; in other words, you must be able to summarize these sources. It will frequently be helpful for your readers if you provide at least partial summaries of sources in your synthesis essays. At the same time, you must go beyond summary to make judgments - judgments based, of course, on your critical reading of your sources - as you have practiced in your reading responses and in class discussions. You should already have drawn some conclusions about the quality and validity of these sources; and you should know how much you agree or disagree with the points made in your sources and the reasons for your agreement or disagreement.

Further, you must go beyond the critique of individual sources to determine the relationship among them. Is the information in source B, for example, an extended illustration of the generalizations in source A? Would it be useful to compare and contrast source C with source B? Having read and considered sources A, B, and C, can you infer something else - D (not a source, but your own idea)?

Because a synthesis is based on two or more sources, you will need to be selective when choosing information from each. It would be neither possible nor desirable, for instance, to discuss in a ten-page paper on the battle of Wounded Knee every point that the authors of two books make about their subject. What you as a writer must do is select the ideas and information from each source that best allow you to achieve your purpose.

PURPOSE
Your purpose in reading source materials and then in drawing upon them to write your own material is often reflected in the wording of an assignment. For example, your assignment may ask that you evaluate a text, argue a position on a topic, explain cause and effect relationships, or compare and contrast items. While you might use the same sources in writing an argumentative essay as your classmate uses in writing a comparison/contrast essay, you will make different uses of those sources based on the different purposes of the assignments. What you find worthy of detailed analysis in Source A may be mentioned only in passing by your classmate.

USING YOUR SOURCES
Your purpose determines not only what parts of your sources you will use but also how you will relate them to one another. Since the very essence of synthesis is the combining of information and ideas, you must have some basis on which to combine them. Some relationships among the material in you sources must make them worth sythesizing. It follows that the better able you are to discover such relationships, the better able you will be to use your sources in writing syntheses. Your purpose in writing (based on your assignment) will determine how you relate your source materials to one another. Your purpose in writing determines which sources you use, which parts of them you use, at which points in your essay you use them, and in what manner you relate them to one another.


TWO TYPES OF SYNTHESES

THE EXPLANATORY SYNTHESIS: An explanatory synthesis helps readers to understand a topic. Writers explain when they divide a subject into its component parts and present them to the reader in a clear and orderly fashion. Explanations may entail descriptions that re-create in words some object, place, event, sequence of events, or state of affairs. The purpose in writing an explanatory essay is not to argue a particular point, but rather to present the facts in a reasonably objective manner. The explanatory synthesis does not go much beyond what is obvious from a careful reading of the sources. You will not be writing explanatory synthesis essays in this course. However, at times your argumentative synthesis essays will include sections that are explanatory in nature.

THE ARGUMENT SYNTHESIS: The purpose of an argument synthesis is for you to present your own point of view - supported, of course, by relevant facts, drawn from sources, and presented in a logical manner. The thesis of an argumentative essay is debatable. It makes a proposition about which reasonable people could disagree, and any two writers working with the same source materials could conceive of and support other, opposite theses.


STANDARDS FOR SYNTHESIS ESSAYS

1. Remember that you are using your sources to support your ideas and claims, not the other way around.

2. Keep in mind that original thought and insightful analysis are required for a 4.0, 3.5, or 3.0 paper; 2.5 and below evaluations tend not to present original ideas.

3. A 4.0, 3.5, or 3.0 paper will create a "dialogue" between the essay author's ideas and her sources, and also among the sources themselves. 2.5 and below evaluations will often summarize one point at a time, with the essay author's idea stated at the end. If you imagine a synthesis essay as a room in which the synthesis writer is joined by the authors of her/his sources, the 4.0, 3.5, or 3.0 essay has everyone engaged in conversation or debate, with everyone commenting on (or arguing against) each other's ideas directly. In the 2.5 and below essay, each person in the room stands up in turn, gives a speech, and sits down, with little or no question and answer period in between or afterward.

4. Take special care to address your audience in an appropriate manner. Make sure you establish your credibility on the subject and that you provide sufficient information to make your argument (thesis) convincing.

5. Organize your paper logically:
A. State your thesis clearly and make sure that it reflects the focus of your essay.
B. Make sure your main points are clearly stated (use topic sentences), and connect each point to your thesis as explicitly as possible.
C. Divide paragraphs logically.
D. Provide appropriate transitions both within and between paragraphs.
6. Develop each main idea thoroughly. Use specific examples and source materials appropriately as support. Be sure to integrate source materials smoothly into your own writing using attribution phrases and transitions. Also be sure to avoid unnecessary repetition (repetition is often an organization problem).

7. Select words precisely. When in doubt, use a dictionary!

8. Make sure sentences are clear and unambiguous. Avoid passive voice. Double-check to see that sentences are adequately varied in length and style, and that there are no fragments or run-ons. Also proofread carefully to correct any other sentence errors.

9. Proofread carefully to identify and correct mechanical errors, such as errors in plurals or possessives, subject-verb agreement, shifts in verb tense or person ("you"), comma errors, spelling errors, and so on.

10. Quadruple check your MLA documentation. Are your parenthetical citations correct? Is your Works Cited list correct according to MLA style, and does it include all sources cited in your essay?

11. Be sure to give your essay a descriptive and attention-getting title (NOT "Synthesis," for goodness sake!!!).

12. Make sure your essay is formatted correctly and posted to your web site correctly.


HOW TO WRITE SYNTHESIS ESSAYS

  1. Consider your purpose in writing. Read the topic assignment carefully. What are you trying to accomplish in your essay? How will this purpose shape the way you approach your sources?
  2. Select and carefully read your sources, according to your purpose. Re-read the sources, mentally summarizing each. Identify those aspects or parts of your sources that will help you in fulfilling your purpose. When rereading, label or underline the passages for main ideas, key terms, and any details you want to use in the synthesis.
  3. Formulate a thesis. Your thesis is the main idea that you want to present in your synthesis. It must be expressed as a complete sentence and include a statement of the topic and your assertion about that topic. Sometimes the thesis is the first sentence, but more often it is the final sentence of the first paragraph.
  4. Decide how you will use your source material and take notes. How will the information and the ideas in your sources help you to fulfill your purpose? Re-read your sources and write down the information from your sources that will best develop and support your thesis.
  5. Develop and organizational plan, according to your thesis. (See Techniques for Developing Synthesis Essays immediately below.) How will you arrange your material? It is not necessary to prepare a formal outline, but you should have some plan in mind that will indicate the order in which you will present your material and that will indicate the relationships among your sources.
  6. Write the first draft of your synthesis, following your organizational plan. Be flexible with your plan, however, and allow yourself room to incorporate new ideas you discover as you write. As you discover and incorporate new ideas, re-read your work frequently to ensure that your thesis still accounts for what follows and that what follows still logically supports your thesis.
  7. Document your sources. Use MLA-style in-text citations and a Works Cited list to credit your sources for all material you quote, paraphrase, or summarize. For example, if I wanted to note in my essay the difference between name-calling and argumentum ad hominem as personal forms of attack, I would credit the article on "Politics: The Art of Bamboozling" fromWARAC by offering a citation that includes the author's last name and the exact page number where she discussed this notion (Cross 302). At the end of the essay, I would have a complete bibliographic citation for the "Politics" article.
  8. Revise your synthesis. Insert transitional words and phrases where necessary. Integrate all quotations so they flow smoothly within your own sentences. Use attribution phrases to distinguish between your sources' ideas and your own ideas. Make sure the essay reads smoothly, logically, and clearly from beginning to end. Check for grammatical correctness, punctuation, and spelling.

TECHNIQUES FOR DEVELOPING SYNTHESIS ESSAYS

SUMMARY: The simplest - and least sophisticated - way of organizing a synthesis essay is to summarize your most relevant sources, one after the other, but generally with the most important source(s) last. The problem with this approach is that it reveals little or no independent thought on your part. Its main virtue is that it at least grounds your paper in relevant and specific evidence.

Summary can be useful - and sophisticated - if handled judiciously, selectively, and in combination with other techniques. At some time you may need to summarize a crucial source in some detail. At another point, you may wish to summarize a key section or paragraph of a source in a single sentence. Try to anticipate what your reader needs to know at any given point of your paper in order to comprehend or appreciate fully the point you are making.

EXAMPLE OR ILLUSTRATION: At one or more points in your paper, you may wish to refer to a particularly illuminating example or illustration from your source material. You might paraphrase this example (i.e., recount it, in some detail, in your own words), summarize it, or quote it directly from your source. In all these cases, of course, you would properly credit your source.

TWO (OR MORE) REASONS: The "two reasons" approach can be an extremely effective method of development. You simply state your thesis, then offer reasons why the statement is true, supported by evidence from your sources. You can advance as many reasons for the truth of your thesis as needed; but save the most important reason(s) for last, because the end of the paper is what will remain most clearly in the reader's mind.

STRAWMAN: When you use the strawman technique, you present an argument against your thesis, but immediately afterward you show that this argument is weak or flawed. The advantage of this technique is that you demonstrate your awareness of the other side of the argument and show that you are prepared to answer it. The strawman argument first presents an introduction and thesis, then the main opposing argument, a refutation of the opposing argument, and finally a positive argument.

CONCESSION: Like the strawman, the concession technique presents the opposing viewpoint, but it does not proceed to demolish the opposition. Instead, it concedes that the opposition has a valid point but that, even so, the positive argument is the stronger one. This method is particularly valuable when you know your reader holds the opposing view.

COMPARISON AND CONTRAST: Comparison and contrast techniques enable you to examine two subjects (or sources) in terms of one another. When you compare, you consider similarities. When you contrast, you consider differences. By comparing and contrasting, you perform a multifaceted analysis that often suggests subtleties that otherwise might not have come to your attention.

To organize a comparison/contrast analysis, you must carefully read sources in order to discover significant criteria for analysis. A criterion is a specific point to which both of your authors refer and about which they may agree or disagree. The best criteria are those that allow you not only to account for obvious similarities and differences between sources but also to plumb deeper, to more subtle and significant similarities and differences. There are two basic formulas for comparison/contrast analysis:

 BY SOURCE
 BY CRITERIA
I. Introduce essay, state thesis I. Introduce essay, state thesis
II. Summarize passage A II. Introduce Criterion 1
  A. View on Criterion I  A. Passage A's viewpoint
  B. View on Criterion 2  B. Passage B's viewpoint
III. Summarize passage BIII. Introduce Criterion 2
  A. View on Criterion 1  A. Passage A's viewpoint
  B. View on Criterion 2  B. Passage B's viewpoint
IV. Discussion and conclusionIV. Discussion and conclusion

Citation: Wallis RS, Dawson R, Friedrich SO, Venter A, Paige D, Zhu T, et al. (2014) Mycobactericidal Activity of Sutezolid (PNU-100480) in Sputum (EBA) and Blood (WBA) of Patients with Pulmonary Tuberculosis. PLoS ONE 9(4): e94462. https://doi.org/10.1371/journal.pone.0094462

Editor: Sarah L. Pett, Faculty of Medicine, Australia

Received: October 21, 2013; Accepted: March 10, 2014; Published: April 14, 2014

Copyright: © 2014 Wallis et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This study was funded by Pfizer. The funder Pfizer provided support in the form of salaries for authors RSW, DP, TZ, AS, JG, CE, YZ and PM but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.

Competing interests: The authors have read the journal's policy and have the following conflicts: RSW, DP, TZ, AS, JG, CE, YZ and PM are/were Pfizer employees, and/or shareholders at the time the study was conducted, whose company funded this study. KE of the University of Arkansas was a paid consultant to Pfizer Inc in regards to the study design. Rights to sutezolid were acquired by Sequella, Inc. in 2013. There are no further patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Introduction

Mycobacterium tuberculosis resistant to current first-line anti-tuberculosis agents is a serious and growing global health threat, causing at least 444,000 new tuberculosis (TB) cases and 150,000 deaths annually [1]. Treatments for drug-resistant (DR) TB are inferior to those for drug-sensitive disease, with lower cure rates, reduced safety and tolerability, and prolonged treatment requirements. Oxazolidinone antimicrobials are increasingly viewed as candidates for inclusion in new regimens for DR-TB, as they have a distinct mechanism of action (binding to the 23S ribosome, thereby blocking microbial protein synthesis) without cross-resistance to existing TB drugs. However, in the case of linezolid, the only currently licensed oxazolidinone, serious hematologic and neurologic toxicities arise during long term use due to inhibition of mitochondrial protein synthesis that often require dose reduction or discontinuation [2], [3]. Thus, for the promise of oxazolidinones for TB to be realized, new drugs in this class with superior efficacy and reduced toxicity must be developed.

Sutezolid (PNU-100480) is a thiomorpholinyl analog of linezolid with preliminary evidence for superior efficacy against M. tuberculosis. In the mouse model, sutezolid shortens standard treatment by 1 month, whereas linezolid does not [4]; in the whole blood culture model, the maximal bactericidal activity of sutezolid (−0.42 log/day) is more than twice that of linezolid (−0.16 log/day, P<0.001) [5]. Time-dependent killing has been reported in whole blood and hollow fibers [5], [6]. Bactericidal activity against intracellular mycobacteria is mainly due to the parent (PNU-100480), whereas a sulfoxide metabolite (PNU-101603) contributes significantly to activity against extracellular mycobacteria [6]. Phase 1 studies revealed no abnormal hematologic or biochemical findings, nor instances of peripheral or ophthalmic neuropathy, in healthy volunteers administered sutezolid 600 mg twice daily for 28 days [5], potentially indicating a safety profile superior to similarly dosed linezolid.

In the present study, sutezolid was administered at doses of 600 mg twice daily or 1200 mg once daily for 14 days to patients with newly diagnosed drug-sensitive pulmonary tuberculosis, to assess its safety, tolerability, pharmacokinetics, and early bactericidal activity (EBA) in sputum. The main objective of the study was to determine if sutezolid treatment resulted in significant reduction in log sputum CFU counts with 5%–95% confidence intervals that excluded zero. The study also examined bactericidal activity in ex vivo whole blood cultures infected with M. tuberculosis (whole blood bactericidal activity or WBA), as well as resistance prevention in sputum (measured as changes in MICs during the treatment period).

Methods

Protocol

The protocol for this trial and supporting CONSORT checklist are available as supporting information; see Checklist S1 and Protocol S1.

Subjects

All subjects provided written informed consent according to ICH guidelines. Subjects consisted of men and women aged 18–65 years, with chest radiographs consistent with pulmonary tuberculosis, positive sputum acid-fast smears, culture or molecular confirmation of drug-susceptible M. tuberculosis, random blood glucose <150 mg/dL, hemoglobin >8 g/dL, serum creatinine <2 mg/dL, AST <3x ULN, total bilirubin <1.3 mg/dL. Serologic testing was not performed for hepatitis B or C, nor were these exclusion criteria. Subjects were either HIV-1 uninfected, or HIV-1 infected with CD4 T cell counts >350/mm3 and not currently receiving anti-retroviral therapy. Patients receiving monoamine oxidase (MAO) inhibitors, tricyclic antidepressants, or adrenergic agonists such as pseudoephedrine or phenylpropanolamine within the preceding 7 days were excluded (due to potential concerns regarding MAO-A inhibition), as were patients who had received drugs with anti-tuberculosis activity within the preceding 6 months, had a positive test for urinary isoniazid metabolite at the time of screening, or who had significant hemoptysis.

Treatments

After providing written informed consent, subjects were randomly assigned in blocks of 7–9 to sutezolid 600 mg BID (N = 25) or 1200 mg QD (N = 25), or to a positive control of weight adjusted fixed dose combination tablets (Rifafour© e275; Sanofi-Aventis, Midrand, South Africa) consisting of isoniazid, rifampin, pyrazinamide, and ethambutol (HRZE, N = 9). The purpose of this group was to ascertain comparability of laboratory methods to other EBA trials. Sutezolid was administered as 200 mg tablets. All treatments were administered on an inpatient basis. Neither subjects nor investigators were blinded to assigned treatment. Laboratory staff performing the sputum and whole blood assays was unaware of treatment allocation. After discharge all subjects were referred for a full course of standard antituberculosis treatment.

Evaluations

Symptoms and physical exam were monitored daily. Electrocardiograms were obtained at baseline, day 1, and day 14. Routine blood and urine safety tests were monitored on days 1, 14, and 42. Pooled sputum samples were collected for 16 hrs on days −1, 0, 1, 2, 3, 4, 6, 8, 10, 12, and 14, beginning in the afternoon of the specified study day and continuing through the next morning. Days −1 and 0 were considered baseline days for statistical calculations. Treatment began in the morning of day 1, after collection of the day 0 sputum sample was completed. Sputum specimens were stored on ice at the bedside during collection and transported to the central laboratory (Department of Biomedical Sciences, Stellenbosch University, Tygerberg, South Africa) at 4°C the next morning. Pooled specimens were homogenized by stirring for 30 minutes, then digested with an equal volume of Sputasol (Oxoid, Cambridge UK) at a final dithiothreitol concentration of 10%. An aliquot was removed and a series of 10-fold dilutions prepared in saline with Tween 80 for log CFU determination on 7H11 agar with Selectatab (polymyxin B, ticarcillin, amphotericin B, trimethoprim, MAST, Bootle, Merseyside, UK) added. Log CFU determinations were performed on specimens collected on days −1, 0, 1, 2, 4, 6, 10, and 14. A second aliquot was decontaminated with 1% NaOH-N-acetyl-L-cysteine, diluted with PBS and centrifuged at 4°C and 3000 g for 15 minutes. The supernatant was discarded and the pellet resuspended in 1.5 ml PBS. 500 µl of this was used to inoculate a Mycobacteria Growth Indicator Tube (MGIT, Becton Dickinson, Sparks, USA) supplemented with OADC (oleic acid, albumin, dextrose, catalase) and PANTA (polymyxin B, amphotericin B, nalidixic acid, trimethoprim, azlocillin). MGITs were incubated at 37°C in a BACTEC MGIT 960 instrument until flagged positive or for a maximum of 42 days in case no growth was detected. Time to positivity (TTP) in MGIT cultures was recorded for all specimens. Contamination was excluded by placing one drop of positive liquid culture on a blood agar plate (NHLS, Cape Town, South Africa) and by incubating for 48 hours at 37°C without visible growth. Contaminated cultures were excluded from analysis.

Blood was collected for WBA on prior to treatment on day 1. Blood was collected for PK and WBA 8 and 12 hrs post dose on day 13, and at 0, 1, 2, 3, and 6 hrs post dose on day 14. Plasma was separated immediately after collection and stored at −20°C for PK determinations. Total plasma concentrations of PNU-100480 and PNU-101603 were determined using a validated LC-MS/MS method by Advion BioServices (Ithaca, NY, USA), as previously described [7]. Blood for WBA was stored at room temperature with slow constant rotation until a full set of samples for a subject had been collected, at which time they were transported to the laboratory. WBA against M. tuberculosis H37Rv was determined as previously described [7]. Briefly, M. tuberculosis H37Rv was grown in MGIT and frozen in aliquots at −80C. A titration experiment determined the relationship between inoculum size and TTP, and identified the volume positive in 5.5 days. Whole blood cultures consisted of heparinized blood, an equal volume of RPMI 1640 tissue culture medium (Highveld Biological, Lyndhurst, South Africa), and mycobacteria from the specified volume of mycobacterial stock. After 72 hrs, cells were sedimented, the liquid phase removed, and blood cells disrupted by hypotonic lysis. Bacilli were recovered and inoculated into MGIT and incubated until flagged as positive. Log change in viability was calculated as log(final) – log(initial), where final and initial are the volumes corresponding to TTP of the completed cultures and its inoculum control, respectively, based on the titration curve. Results were expressed as log change per day of whole blood culture. Cumulative WBA over 24 hrs was calculated as the AUC0-24, and expressed as Δlog/d•d, or simply as log change.

Minimal inhibitory concentration (MIC) testing

MICs of sutezolid and its major metabolite were determined using MGIT. Sterile stock solutions of sutezolid and PNU-101603 10 mg/ml were prepared in DMSO. Testing was performed using a series of 2-fold reductions in drug concentrations from 4.0 to 0.062 µg/ml. Growth in drug-containing tubes was compared to that of a positive growth control in which the inoculum was diluted 1∶100 in saline. The MIC was defined as the lowest concentration of drug without growth (GU<100) at the time when growth was detected in the positive control.

Safety reporting

Specific guidance was provided to investigators for the identification of serious adverse events (SAEs), which were defined as those resulting in death, being life-threatening, requiring or prolonging hospitalization, resulting in persistent or significant disability or incapacity, or resulting in congenital anomaly. Guidance was provided for classification of abnormal test results as adverse events and as well as criteria for laboratory abnormalities of potential clinical concern. Potential cases of drug-induced liver injury were defined by Hy's criteria [8]. AE severity was clinically assessed as mild, moderate, or severe according to the extent to which it interfered with the subject's usual function (none, partially, or significantly). The accuracy of all entered study data was verified by independent study monitors.

Statistics

The sample size for the sutezolid arms was selected to have 80% power to detect a change from baseline in sputum log10 CFU/mL from baseline to day 2 of −0.18 (equal to that observed for linezolid 600 mg QD), using a one-sided test at the 0.05 significance level, and assuming between subject variability (SD) of 0.35 [9], [10]. Sputum log10 CFU/ml was analyzed by mixed effects model repeated measures analysis (MMRM), using fixed effects for treatment, baseline values, study day as a categorical variable, and study day by treatment interaction, and a random effect for subject with unstructured within subject covariances (unstructured to allow for its full estimation from present data). Rates of change from baseline to day 2, from day 2 to 14, and from baseline to day 14 were examined. Additional analyses of sputum log10 CFU/ml were conducted using ANCOVA of change from beginning to end of each period, using ANCOVA of changes based on regression slope, and using mixed effects model repeated measures analysis with day as a regression variable. 90% confidence limits (5%–95%) were calculated to be consistent with a one-sided test at the 0.05 significance level. Changes in MGIT TTP were analyzed similarly. Cumulative WBA was analyzed by an ANCOVA with fixed effects for treatment and baseline, and a random effect for subject. MIC results were analyzed post-treatment vs. pre-treatment post hoc, separately for parent/metabolite and BID/QD schedules due to different characteristics and data distributions, by generalized linear mixed model repeated measures analysis (GLMMRM), using multinomial cumulative logit link with proportional odds. GLMMRM is potentially a more sensitive test in the case of missing data. A nonparametric sign test was also performed post hoc on MIC results as a sensitivity analysis, as a more conservative method. Two-sided testing was used on these post hoc analyses. There was no multiplicity adjustment for tests in different datasets.

Ethical review

This study received ethical approval from the University of Cape Town Faculty of Health Sciences Human Research Ethics Committee, Cape Town, South Africa, and from Pharma-Ethics, Lyttelton Manor, South Africa. The study received regulatory approval in South Africa and was registered on clinicaltrials.gov as NCT01225640.

Role of the funding source

This study was funded by Pfizer, Inc. Pfizer employees contributed to study design, data analysis, and data interpretation. All authors had full access to all study data and had final responsibility for the decision to submit for publication. Rights to sutezolid were acquired by Sequella, Inc. in 2013.

Results

Subjects

Enrollment began at both sites in August 2011; study participation was completed by December 2011. A CONSORT flow diagram showing enrollment is shown in figure 1. Subject demographic and baseline characteristics were similar among the 3 arms (table 1). Subjects were primarily male (80%) and <45 years of age (81%). Four subjects were HIV-1 seropositive, with CD4 T cell counts ranging from 643 to 812 cells/µl. The baseline sputum bacillary burden was approximately 107 CFU/ml, similar to other EBA trials. All subjects completed their assigned treatments, began subsequent standard TB treatment without interruption, and were included in the full analysis set.

Sputum bactericidal activity

Both dosing schedules of sutezolid resulted in changes in sputum log10 CFU over the entire period of treatment that excluded zero (table 2 and figure 2 left panel). Separate analysis of days 0–2 and 2–14 showed the effect only was significant during the later interval. A trend toward a superior effect was apparent in mean values across days when sutezolid was given as 600 mg BID (figure 2), but the confidence intervals for the two dosing schedules largely overlapped (figure 2 and table 2). Effects in HRZE-treated subjects were similar to those reported in other EBA trials [11].

Figure 2. Bactericidal activity in sputum according to treatment arm as assessed by colony counts (left) and time to positivity in automated liquid culture (MGIT TTP, right).

Lines indicate prediction and shading 90% confidence interval (CI) as determined by mixed effects model repeated measures analysis, using day as a categorical variable. The vertical axis of the right hand figure is inverted to facilitate visual comparison with CFU findings. At 14 days, the 90% CI of all treatments excluded zero.

https://doi.org/10.1371/journal.pone.0094462.g002

Table 2. Rate of change of sputum log10 CFU counts per day according to mixed effects modeling repeated measures analysis, using fixed effects for treatment, day, treatment by day interaction, and baseline values (defined as the mean of days −1 and 0).

https://doi.org/10.1371/journal.pone.0094462.t002

Changes in MGIT TTP analyzed by MMRM are illustrated in the right panel of figure 2. Like CFU counts, the 90% CI of the effects on TTP of both dosing schedules excluded zero. However, unlike CFU counts, no lag period during early treatment was apparent (i.e., changes from baseline to day 2 were statistically significant), nor was any trend apparent toward superiority of 600 mg BID dosing.

Whole blood bactericidal activity

Prior to the start of treatment, there was mean growth of M. tuberculosis H37Rv of approximately +0.2 log/d in ex vivo whole blood cultures (table 1), corresponding to a doubling time of approximately 36 hrs. Sutezolid treatment resulted in readily detectable bactericidal activity whether administered as a single or divided dose (figure 3). Both doses produced maximal killing of approximately −0.4 log/d, occurring 2–3 hrs post dose. Analysis of cumulative activity over 24 hr by ANCOVA indicated log10 estimates of -0.142±0.020 and −.089±0.020 (mean ± SE) for BID and QD dosing, respectively. Although the 90% CI of difference between the 2 schedules excluded zero (−0.100 to −0.005), the difference only approached significance at the two-tailed 5% level (P = 0.068). There was no correlation between cumulative WBA and EBA from baseline to day 2 or from days 2–14 among sutezolid-treated patients (all R<0.2, P>0.2).

Figure 3. Bactericidal activity against M. tuberculosis H37Rv in whole blood culture (WBA) according to treatment arm, at discrete time points (left), and as cumulative effect (right).

Lines and shading indicate means and 90% confidence intervals (CI).

https://doi.org/10.1371/journal.pone.0094462.g003

Minimum inhibitory concentrations

MIC testing of sutezolid and its major metabolite was incomplete, lacking 38/200 (19%) results due to time and resource constraints. Median MIC values for the parent and metabolite at baseline were ≤0.062 µg/ml and 0.500 µg/ml, respectively, in both treatment arms. Testing was repeated on day 15 to determine if values increased due to treatment. In patients treated with sutezolid 1200 mg QD, the median MIC of the parent on day 15 was 0.125 µg/ml. This reflected 5 instances in which MICs increased vs. none in which they decreased (figure 4). However, the significance level of this change (two-tailed GLMMRM: P = .034; two-tailed sign test: P = .063) does not account for the 4 comparisons conducted for the 4 different datasets (2 treatment arms, parent and metabolite) in post-hoc testing. All other median MICs were unchanged (P≥.727).

Figure 4. Distributions of minimal inhibitory concentrations (MICs) to sutezolid (PNU-100480) pre and post treatment (rows and columns, respectively), according to dosing schedule.

Values in each cell indicate numbers of patients. Cells shaded red are those in which MIC values increased, whereas they decreased in those shaded blue. No change was apparent in MIC values of the metabolite (PNU-101603, not shown).

https://doi.org/10.1371/journal.pone.0094462.g004

Plasma pharmacokinetics

Plasma concentrations of sutezolid and its major metabolite (PNU-101603) on day 14 are shown in figure 5. Key PK parameters are summarized in table 3. While the AUC0−24 values were comparable between the QD and BID dosing regimens for both sutezolid and PNU-101603, administration of sutezolid as a single daily dose resulted in a doubling of the Cmax but slightly less than a doubling of that of the major metabolite. When dosed at 600 mg BID, median plasma concentrations of parent and major metabolite remained above their respective median MIC values for 71% and 89% of the dosing interval. In contrast, dosing at 1200 mg QD resulted in median supra-MIC plasma concentrations for 53% and 57% of the dosing interval, respectively. The observed mean AUC0–24 of parent and metabolite appeared to range from 63–71% and 86–91%, respectively, of values observed in phase 1, whereas Cmax values of parent and metabolite equaled those observed in healthy volunteers in phase 1 [5].

Figure 5. Plasma concentrations of sutezolid (pink) and its major metabolite (yellow) at steady state (day 13–14) in patients treated with sutezolid 600 mg BID (left) or 1200 mg QD (right).

Solid lines indicate medians; shading indicates 90% CI. Lower and upper dotted lines indicate median pre-treatment MIC values for parent and metabolite, respectively.

https://doi.org/10.1371/journal.pone.0094462.g005

Safety

Treatment with sutezolid was generally safe and well tolerated. No subject required dose reduction or premature discontinuation due to adverse events or abnormal laboratory parameters. There were no instances of anemia or thrombocytopenia. There was no effect of sutezolid on the QTc interval, with changes from baseline to day 14 of −4.2±14.5 msec and −3.1±12.1 msec (mean±SD) in the 600 mg BID and 1200 mg QD arms, respectively. Treatment-emergent adverse events were distributed evenly across the sutezolid arms and were mainly classified as mild (n = 26) or moderate (n = 13) in severity. A total of 7 sutezolid-treated subjects (14%) experienced mild or moderate increases in alanine transaminase (ALT). Cases were distributed in both men and women (5∶2) and in both the BID and QD treatment arms (4∶3). None occurred in HIV-1 seropositive individuals. No other predisposing factors were identified. ALT values for these subjects increased from 34±24 IU/L at baseline (mean±SD) to 173±34 IU/L on day 15 (reference range, 6–48 IU/L) (figure 6). ALT increases were accompanied by smaller increases in AST, but not by changes in alkaline phosphatase or bilirubin. None of the subjects experienced symptoms of drug-induced liver injury. None met Hy's criteria for serious liver injury [8]. All 7 subjects completed their assigned sutezolid treatment and began standard TB therapy without interruption. Five of the 7 subjects were recalled for repeat testing on day 22, by which time values had declined to 75±28 IU/L. Values for all patients had returned to normal on day 42 (24±9 IU/L).

One adverse event met study criteria for both serious and severe: hemoptysis occurred in 1 subject 14 days after the last dose of sutezolid 600 mg BID. It was thought by both the investigator and sponsor to be related to tuberculosis rather than its treatment. Lastly, one instance of grade 1 peripheral neuropathy occurred in a patient assigned to sutezolid 600 mg BID. However, its onset occurred after sutezolid treatment had been completed, and was attributed by the investigator to the standard TB treatment that followed. A listing of all treatment-emergent adverse events of all potential causes can be found in table S1.

Discussion

This is the first study of sutezolid in patients with pulmonary tuberculosis. The main findings were that doses of 600 mg BID and 1200 mg QD given for 14 days were generally safe, well tolerated, and resulted in readily detectable bactericidal activity in both sputum and blood. Its effects in sputum were sustained throughout the full period of treatment. These findings support further development of sutezolid as a component of new tuberculosis regimens.

Prior studies have separately examined the mycobactericidal activity of linezolid in sputum and blood. One study in a total of 19 TB patients found linezolid 600 mg BID and 600 mg QD produced effects approaching statistical significance of −0.26 and −0.18 log10 CFU/ml/day, respectively, during the first 2 days of treatment, but not subsequently [9]. In contrast, the present study found no significant effect on sputum CFU counts during the first 2 days of treatment, but did find a significant, sustained effect subsequently. Effects during early TB treatment are thought to represent activity against extracellular, metabolically active bacilli in cavities. This bacillary subpopulation is critical for TB transmission and for the emergence of resistance, but it appears not to be involved in persistence and relapse. The sustained activity of sutezolid observed in the present trial may indicate enhanced ability to sterilize tissues and thereby shorten the required duration of treatment, as has been shown in the mouse model [4]. However, there is no evidence at present to indicate a relationship between EBA during the first 2 weeks of TB treatment and the total duration required for relapse-free cure [12].

This trial is the first in which intracellular bactericidal activity in blood of TB patients has been assessed in parallel with that in sputum. Sutezolid and its main metabolite appear to act against distinct mycobacterial subpopulations. In vitro, extracellular killing is mainly due to the metabolite, whereas killing of intracellular mycobacteria, such as in the whole blood model, is mainly due to the parent [13], [14]. Sutezolid shows superior activity vs. linezolid in the whole blood model [5]. In one study, whole blood bactericidal activity during TB treatment correlated with 2 month sputum culture status [15], which in turn is a predictor of relapse [16]. Longer trials will be required to better assess the sterilizing activity of sutezolid and to determine the required duration of new sutezolid-containing regimens. Future trials may also consider studying each patient using his or her own isolate in whole blood culture to enhance its predictive value [15].

The mycobactericidal activity of sutezolid in the whole blood and hollow fiber models is mainly dependent on time rather than concentration [5], [13]. Given this observation, the relatively short plasma half-lives of both the parent and metabolite (approximately 4 hrs) ordinarily would favor divided rather than single daily dosing. In the present study, divided dosing tended to show superior bactericidal activity in blood, although this at best was only at the threshold of statistical significance. This trial was neither intended nor powered to detect a difference between single and divided daily dosing. Longer clinical trials will be required to determine the potential clinical impact of single vs. divided daily dosing when sutezolid is combined with other TB drugs in novel regimens.

Prevention of resistance is an essential characteristic of TB drugs. Experience with linezolid indicates this characteristic may be distinct from EBA, as linezolid lacks sustained EBA [9], yet shows remarkable ability to prevent acquired resistance even when added as a single new drug to a failing regimen [3]. No conclusion can be drawn regarding the MIC findings in this trial due to the post-hoc nature of the analysis and the relatively small magnitude of observed increases. However, EBA trials are intended to closely study a small number of subjects during a short period of treatment so as to inform the design of future trials. Studies of sutezolid in the hollow fiber model have reported similarly reduced bactericidal activity and earlier emergence of resistance with simulated once daily dosing [6]. Pre- and post-treatment MIC testing should be considered to assess resistance prevention in future EBA trials.

Peak concentrations of sutezolid and its main metabolite in TB patients in this study equaled those observed in previous studies in healthy volunteers, whereas total exposure to the parent was reduced by one-quarter. This decreased exposure appears to be the cause of the reduced bactericidal activity in whole blood cultures at later time points in patients as compared to those previously reported in healthy volunteers [5]. Macrophage production of oxygen radicals is increased in TB as part of the antimycobacterial host response. Non-enzymatic oxidation may contribute to sutezolid metabolism. Further studies are warranted to determine whether the rate of metabolic clearance of sutezolid slows as inflammation resolves during TB treatment, as this may restore intracellular bactericidal activity to levels observed in volunteers.

Mild to moderate increases in hepatic alanine aminotransferase were observed in 14% of sutezolid-treated subjects in this study. In contrast, ALT levels had remained within the normal range in all subjects during phase 1 studies of sutezolid in healthy volunteers. Early, mild, transient, asymptomatic increases in liver enzymes are common in patients treated with anti-TB drugs [17]. There is increasing evidence that these represent heightened susceptibility to oxidative liver injury due to depletion of glutathione, which ordinarily protects against such damage. Intracellular stores of glutathione are depleted, and the ratio of reduced to oxidized glutathione diminished, following experimental M. tuberculosis infection of guinea pigs [18]. Glutathione levels are low in TB patients, with the lowest levels occurring in those patients who go on to experience TB drug-induced liver injury [19]–[21]. Cysteine availability is the limiting step in glutathione synthesis. A small prospective randomized trial reported that TB drug-induced liver injury could be prevented by supplementation with N-acetylcysteine (NAC) [22]. A study of NAC may be considered to reduce overall risk in future trials of new TB drugs, including sutezolid.

In summary, this first trial of the oxazolidinone sutezolid in patients with pulmonary tuberculosis found the drug to be generally safe, well tolerated, and with readily detectable bactericidal activity in sputum and blood. Further studies of this promising compound are warranted.

Author Contributions

Conceived and designed the experiments: RSW RD AV SF DP TZ AS JG CE YZ KE PM AHD. Performed the experiments: RD AV SF AHD. Analyzed the data: YZ TZ RSW. Contributed reagents/materials/analysis tools: RSW. Wrote the paper: RSW RD AV SF DP TZ AS JG CE YZ KE PM AHD.

References

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